Components

Antigen Control Slide - (Catalog No. Adeno - 5009, Influenza A & B - 5010, Para 1,2,3 - 5011, RSV - 5012). Four control slides containing wells with positive and negative control cells. One positive control well each is infected with the following: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV. Negative control wells contain uninfected cells.Monoclonal Antibodies - (Catalog No. Adeno - 5000, Influenza A - 5001, Influenza B - 5002, Para 1 - 5003, Para 2 - 5004, Para 3 - 5005, RSV - 5006). Seven x 2 ml dropper vials containing monoclonal antibody against: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV.Respiratory Viral Screen - (Catalog No. 5007). One 10 ml dropper vial containing monoclonal antibody against: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and respiratory syncytial virus.Normal Mouse Antibody - (Catalog No. 5014). One 10 ml dropper vial containing normal mouse antibody to be used as a negative control.Anti-Mouse IgG:FITC Conjugate - (Catalog No. 5008). Two 10 ml dropper vials containing FITC labeled goat anti-mouse IgG.PBS - (Catalog No. 5087). One packet of Phosphate Buffered Saline Salts yields 1 liter when dissolved in distilled water. Store in a clean closed container at room temperature.Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One 10 ml vial containing polyoxyethylene sorbitan monolaurate (Tween 20) and sodium azide (NaN3) concentrate to be diluted 1:100 in PBS.Mounting Fluid - (Catalog No. 5013). One 10 ml dropper vial containing Tris- buffer, glycerin, fluorescence enhancer, and sodium azide as preservative. Store at room temperature.

Disclaimer

For in vitro Diagnostic UseCE Mark

General description

Diagnostics Kit

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

10 mL Screen & 2 mL each viral spec. abs.

Storage and Stability

When stored at 2-8°C, the Light Diagnostics Respiratory Panel 1 Viral Screening and Identification Kit is stable up to the expiration date printed on the kit label. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the kit expiration date.

A marked decrease in fluorescence may indicate conjugate or antibody deterioration. A positive control should be tested with each batch of specimens to ensure proper functioning of these reagents and proper staining procedure. If, after appropriate analysis, there is a decrease in staining intensity, discontinue use of the reagents.

Warnings and Precautions:

⋅ Sodium azide (present in the conjugate, monoclonal antibodies, wash buffer, and mounting fluid) can react with lead or copper plumbing to form potentially explosive metal azides. When disposing of these materials, flush with large volumes of water to prevent azide build-up.

⋅ Pooling or diluting conjugates or monoclonal antibodies may cause erroneous results.

⋅ Do not allow slides to dry at any time during the staining procedure.

⋅ Handle all specimens and materials as if potentially infectious. Decontaminate with 0.05% sodium hypochlorite (a 1:100 dilution of household bleach) prior to disposal.

⋅ Avoid prolonged exposure to bright lights.

⋅ Avoid contact with Evans blue (present in the Anti-Mouse IgG : FITC Conjugate) as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

⋅ Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks, or flames. Avoid breathing vapor. Use adequate ventilation.

⋅ Do not mouth pipette reagents.

⋅ Do not substitute reagents from other manufacturers.

⋅ Alteration of protocol provided may cause erroneous results.

⋅ When staining multiple samples on a slide, avoid cross contamination between samples.

⋅ Normal mouse antibody should be tested with each cell culture isolate. Fluorescence indicates a nonspecific reaction and the test is considered invalid.